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Indian Forest Service (Mains)

Chemistry optional

  • 2 papers
  • 200 marks each
  • 3 h per paper

Paper II covers organic chemistry and spectroscopy.

Indian Forest Service (Mains), Chemistry optional · 2025 · Paper II

Q5(a). Edman degradation

Question

Derive the monomeric product identified by HPLC in Edman peptide degradation.

Answer in brief

The identified product is the phenylthiohydantoin (PTH) derivative of the original N-terminal amino acid.

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The full model answer

As written for the answer booklet: the diagram carries the working, the lines stay short. Read here only; it is not offered as a download.

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Topic notes: biopolymers: proteins, peptides, DNA and RNA, with every Civil Services, IFoS and Geo-Scientist PYQ →

CoversEdman degradationphenyl isothiocyanatephenylthiohydantoin (PTH)N-terminal amino acidpeptide sequencing

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